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Additionally, no significant difference was observed in the number of Goblet cells (Alcian blue coloration), Paneth cells (lysozyme immunofluorescence), enteroendocrine cells (Chromogranin A staining) and enterocytes (sucrase-isomaltase expression).We tested the progression of inflammation in Dusp6 KO mice in the acute DSS-colitis model.Integrins are a family of heterodimeric glycoproteins involved in bidirectional cell signaling and participate in the regulation of cell shape, adhesion, migration, differentiation, gene transcription, survival and proliferation.The integrin α1 subunit is known to be involved in RAS/ERK proliferative pathway activation and plays an important role in mammary carcinoma cell proliferation and migration.We finally knocked out DUSP6 in colorectal cancer (CRC) cells (HT29) using Crispr-Cas9 technology.

Increased intestinal permeability (IP) has been observed in a number of autoimmune diseases.

Bacterial DNA extracted from the stool of 1098 healthy subject was sequenced for the V4 hypervariable regions of the 16S r RNA using the Illumina Mi Seq platform.

The function of the fecal microbial communities was then imputed using PICRUSt V0.1 after a rarefaction step to 30,000 sequences per sample.

Among 3,773 KEGG and 3,618 COG functions, we found several nominal associations with IP, the most significant being involved in tyrosine metabolism and degradation of aromatic compounds (K01826), possibly involved in tellurite resistance (COG3615), and DNA uptake process and recombination (COG4469) (Conclusions.

Multivariate analysis controlling for major contributing factors to IP allowed us to identify that individuals currently living with a dog had increased IP.

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